Team:IBB Pune/construct
From 2009.igem.org
(Difference between revisions)
Samitwatve (Talk | contribs) |
|||
Line 12: | Line 12: | ||
[[Image:Tape1.png|center|400px]] | [[Image:Tape1.png|center|400px]] | ||
In this state it encounters a '0' (represented by Lactose) first. In this state, the LuxR protein will be constitutively produced. The LacO (to which repressor protein remains bound in the normal state) becomes activated in presence of Lactose. However this does not lead to the expression of the reporter gene as this requires activation of the pLuxR promoter (requiring AHL). Thus the Turing Machine remains in state 'A', leaves the '0' unchanged and moves one step to the RIGHT. | In this state it encounters a '0' (represented by Lactose) first. In this state, the LuxR protein will be constitutively produced. The LacO (to which repressor protein remains bound in the normal state) becomes activated in presence of Lactose. However this does not lead to the expression of the reporter gene as this requires activation of the pLuxR promoter (requiring AHL). Thus the Turing Machine remains in state 'A', leaves the '0' unchanged and moves one step to the RIGHT. | ||
- | + | [[Image:tape22.png|center|400px]] | |
This process is continues till the Turing Machine reaches the first '1' on the tape, (represented by 'AHL' (acyl homoserine lactone) ). This induces the pLuxR promoter to be switched 'on'. This occurs via the interaction of 'AHL-pLuxR' complex which activates the pLuxR promoter to express the LuxI gene. LuxI gene is responsible for the production of the enzyme Homoserine Lactone Synthase (an enzyme which produces AHL). This is regulated by a positive feedback loop. The AHL which is synthesized by the cells keeps the pLuxR active thus enabling the production of even more AHL. This also has the capacity to activate the other pLuxR promoter present in cassette 2. However in ABSENCE of Lactose, the LacO site has repressor protein bound to it. This prevents the transcription of the reporter gene. | This process is continues till the Turing Machine reaches the first '1' on the tape, (represented by 'AHL' (acyl homoserine lactone) ). This induces the pLuxR promoter to be switched 'on'. This occurs via the interaction of 'AHL-pLuxR' complex which activates the pLuxR promoter to express the LuxI gene. LuxI gene is responsible for the production of the enzyme Homoserine Lactone Synthase (an enzyme which produces AHL). This is regulated by a positive feedback loop. The AHL which is synthesized by the cells keeps the pLuxR active thus enabling the production of even more AHL. This also has the capacity to activate the other pLuxR promoter present in cassette 2. However in ABSENCE of Lactose, the LacO site has repressor protein bound to it. This prevents the transcription of the reporter gene. |
Revision as of 22:46, 21 October 2009