Team:IBB Pune/project/Results

From 2009.igem.org

(Difference between revisions)

Revision as of 00:21, 22 October 2009

We carried out the following transformations successfully:

Native Biobricks

1) pLL promoter - BBa_I746365 2) GFP coding sequence - BBa_E0040 3) RFP coding sequence 4) GFP Translational unit 5) RFP Translational unit 6) Enhanced CFP 7) Fast GFP 8) Constitutive promoter -BBa_J23102 9) Promoter + RBS 10) T7 promoter 11) pLuxR 12) Double terminator 13) RBS 14) Constitutive LuxI 15) LuxR Translational unit

OUR PARTS

1) YcdB 2) TorA 3) Lac Operator

Assembly

1) Const. Promoter-LacO 2) PluxR- LuxI 3) {Promoter + RBS}-GFP 4) {Promoter + RBS}-TorA 5) {Promoter + RBS}-Ycdb 6) luxR-PluxR-{RBS-LuxI} 7) Promoter + RBS 8) YcdB-GFP 9) TorA-GFP 10) {Promoter + RBS}-LuxR 11) {Promoter + RBS}-YcdB-GFP 12) {Promoter + RBS}-TorA-GFP 13) Const. Promoter-LuxR 14)

@@ Line 3: / Line 3: @@
+We carried out the following transformations successfully:
-=Project 2 =
+==Native Biobricks==
+) pLL promoter               - BBa_I746365
+) GFP coding sequence        - BBa_E0040
+) RFP coding sequence
+) GFP Translational unit
+) RFP Translational unit
+) Enhanced CFP
+) Fast GFP
+) Constitutive promoter      -BBa_J23102
+) Promoter + RBS
+) T7 promoter
+) pLuxR
+) Double terminator
+) RBS
+) Constitutive LuxI
+) LuxR Translational unit
+==OUR PARTS==
+) YcdB
+) TorA
+) Lac Operator
+==Assembly==
+) Const. Promoter-LacO
+) PluxR- LuxI
+) {Promoter + RBS}-GFP
+) {Promoter + RBS}-TorA
+) {Promoter + RBS}-Ycdb
+) luxR-PluxR-{RBS-LuxI}
+) Promoter + RBS
+) YcdB-GFP
+) TorA-GFP
+) {Promoter + RBS}-LuxR
+) {Promoter + RBS}-YcdB-GFP
+) {Promoter + RBS}-TorA-GFP
+) Const. Promoter-LuxR
+)