Team:UNICAMP-Brazil/Notebooks/August 11

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====Selection of transformants====
====Selection of transformants====
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Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB medium containing the appropriate antibiotic and let them grow O/N.  
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*<p style=”text-align:justify;”>Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.</p>
==''' YeastGuard '''==
==''' YeastGuard '''==

Revision as of 00:31, 22 October 2009

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ColiGuard

Selection of transformants

  • Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.

YeastGuard

Recovering the biobricks

  • We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.
  • We will need large amounts of DNA so we inoculated colonies in liquid media in order to repeated the plasmid extractions of the six biobricks we are really going to use.


Taís




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