Team:UNICAMP-Brazil/Notebooks/August 18

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====Enzyme restriction====
====Enzyme restriction====
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We made the Mini-prep (according to the protocol 2) and immediately make enzyme restriction with XbaI e PstI in order to prove the part existing in the plasmids. We didn’t find the correct band size (514 bp); we just found the linear plasmid size band.
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*<p style=”text-align:justify;”>We performed miniprep according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2] and immediately made enzymatic digestion using ''Xba''I and ''Pst''I in order to prove the existing part in the plasmids. We didn’t find the correct band size (514 bp); we just found the linear plasmid size band.</p>
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[[Image:18_agosto.jpg‎‎ |center|‎]]

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YeastGuard

Terminator biobrick

  • The biobrick BBa_J63002 was ressuspended from the plate and the transformed E. coli have occupied the whole plates, both LB-AMP and LB-KAN plates. We decided to replicate these colonies in other plates in order to get isolated colonies for the miniprep.

Taís

ColiGuard

Enzyme restriction

  • We performed miniprep according to Protocol 2 and immediately made enzymatic digestion using XbaI and PstI in order to prove the existing part in the plasmids. We didn’t find the correct band size (514 bp); we just found the linear plasmid size band.

‎

Luige





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