Team:UNICAMP-Brazil/Notebooks/September 3

From 2009.igem.org

(Difference between revisions)
(→Terminator Biobrick)
(→MicroGuards)
Line 10: Line 10:
*<p style=”text-align:justify;”>Today we prepared solutions, materials and the pre inoculum to prepare electrocompetent ''E. coli''.</p>
*<p style=”text-align:justify;”>Today we prepared solutions, materials and the pre inoculum to prepare electrocompetent ''E. coli''.</p>
 +
 +
=='''Coliguard'''==
====Hemolysin Operon Amplification====
====Hemolysin Operon Amplification====

Revision as of 01:23, 22 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

MicroGuards

Electrocompetent E. coli

  • Today we prepared solutions, materials and the pre inoculum to prepare electrocompetent E. coli.


Coliguard

Hemolysin Operon Amplification

  • We did the PCR to amplify the hemolysin operon using the total DNA extration that we had made before. Our sequence have almost 4000bp so we used a extension time of 3 minutes, the annelling temperatures in Celsius degrees was 56, 54, 52 and 51. No DNA fragment was shown in the agarose gel.

Marcos

YeastGuard

Terminator Biobrick

  • The terminator plasmid was quantified and we made another digestion, Protocol 14. We found by electrophoresis that it worked!

End.png

Wesley




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/September_3"