Team:UNICAMP-Brazil/Notebooks/August 29
From 2009.igem.org
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=====Material===== | =====Material===== | ||
- | We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of ''Gallus gallus'': the blood, the spleen and the oviduct. =) | + | *<p style=”text-align:justify;”>We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of ''Gallus gallus'': the blood, the spleen and the oviduct. =)</p> |
[[Image:chicken_run_4.jpg|right]] | [[Image:chicken_run_4.jpg|right]] | ||
=====RNA extraction===== | =====RNA extraction===== | ||
- | We used the Trizol protocol for the three tissues ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/RNA_isolation_from_%27Gallus_gallus%27_spleen%2C_blood_and_oviduct Protocol 8]). | + | *<p style=”text-align:justify;”>We used the Trizol protocol for the three tissues ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/RNA_isolation_from_%27Gallus_gallus%27_spleen%2C_blood_and_oviduct Protocol 8]).</p> |
- | We obtained pellets from all tissues! Hope their concentration and quality are ok! =] | + | *<p style=”text-align:justify;”>We obtained pellets from all tissues! Hope their concentration and quality are ok! =] </p> |
''Gleidson, Raíssa, Taís and Sula'' | ''Gleidson, Raíssa, Taís and Sula'' | ||
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====Terminator Biobrick==== | ====Terminator Biobrick==== | ||
- | Today we perfomed miniprep of the Terminator Biobrick transformed ''E. coli'', according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. | + | *<p style=”text-align:justify;”>Today we perfomed miniprep of the Terminator Biobrick transformed ''E. coli'', according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].</p> |
''Wesley'' | ''Wesley'' | ||
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====RBS+CFP==== | ====RBS+CFP==== | ||
- | Today we perfomed miniprep of the RBS+CFP Biobrick transformed ''E. coli'', according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2]. | + | *<p style=”text-align:justify;”>Today we perfomed miniprep of the RBS+CFP Biobrick transformed ''E. coli'', according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].</p> |
''Raíssa and Taís'' | ''Raíssa and Taís'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Latest revision as of 01:53, 22 October 2009
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