Team:Brown/Notebook Meetings/5-27-09
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(New page: {{Brown}} Brown iGEM 2009 Phone Conference 5/27/09 (Pacific Time) 8:20PM: Meeting starts after a lot of technical difficulties 8:22: Brad Lowell at Harvard does not have UCP2, Inv...) |
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- | Brown iGEM 2009 | + | '''Brown iGEM 2009''' |
- | + | ||
- | + | ||
- | + | '''Phone Conference''' | |
- | + | ||
- | + | '''5/27/09''' | |
- | + | ||
- | + | ||
- | + | ||
- | + | '''(Pacific Time)''' | |
- | 8: | + | *8:20PM: Meeting starts after a lot of technical difficulties |
- | 8: | + | *8:22: Brad Lowell at Harvard does not have UCP2, Invitrogen may have it looked up UCPs on invitrogen |
- | + | **What expression vectors has the past iGEM teams used? | |
- | + | **We need E. coli expression vectors | |
- | + | ||
- | 8: | + | *8:32 Ahmad, ask them for the UCP |
- | + | ||
- | 8: | + | *8:29: Indu wrote a nicely worded email. |
- | + | *8:31: Picking holins (there’s 3000!) | |
- | + | **There are some in the registry | |
- | + | **UC Berkeley from last year used Holin | |
- | + | **Ahmad will ask them for a couple Holins | |
- | + | *8:49: CCDB – stops cell growth – can be another redundant mechanism | |
- | + | **It is in the registry | |
- | + | ||
- | + | *8:56: All of the parts so far are in the registry, we need a unique part to submit to the registry | |
- | 9: | + | *9:01: We need to schedule a meeting with the panel for when we get back! |
- | + | **June 17th | |
- | + | **Email the professors | |
- | + | **It would be nice to have Gary Wessel email them. | |
- | 9: | + | *9:08: Michael write an email out to the professors inviting them |
- | + | **Have the draft proofread by the group | |
- | + | **Have Wessel forward it to everybody | |
- | + | ||
- | 9: | + | *9:15: Somebody else – research a tight repressor!! |
- | + | ||
- | 9: | + | *9:17: Indu will follow up phone call the lab for the tick protein |
- | + | **Second idea to the histamine binding protein – have something that also binds IgE antibodies | |
- | + | **This provides redundancy – both binding current histamine and stopping further secretion of histamine | |
- | + | **Link them with glycine bridge | |
- | 9: | + | *9:26: Probiotic system: natural bacteria that secretes protein into bloodstream |
+ | **We have getting it into the system from MIT yogurt | ||
+ | **We have intestinal implantation BactoKidney | ||
+ | **How do we get the bifunctional protein secreted? | ||
- | 9: | + | *9:44: There is a lady named Edith that Will will email – she can help us on probiotic drug transport across organs |
+ | **Ashley, Eli, Will, Minoo can research probiotic | ||
- | + | *9:45: Indu found a paper on protein that binds IGE antibody – she will put it on the google group | |
- | + | **Somebody needs to research IGE | |
+ | **Eli will put up a paper about packaging | ||
+ | **Will is emailing about drug delivery systems | ||
- | + | *9:46: Any paper that may be useful to either project will be put on the google groups | |
- | + | *9:56: Before 8pm PST – everyone go on the google group and build on the research that’s already been done. | |
- | 10:16: bye everybody | + | *10:00: Secretion: Eli, Will, Ashley |
+ | **OK everyone just decide what to research on the google group. | ||
+ | |||
+ | *10:01: We are meeting on SUNDAY MAY 31 SAME TIME (Monday for Minoo) | ||
+ | |||
+ | *10:02: Put up minutes. | ||
+ | |||
+ | *10:16: bye everybody |
Latest revision as of 02:19, 22 October 2009
Brown iGEM 2009
Phone Conference
5/27/09
(Pacific Time)
- 8:20PM: Meeting starts after a lot of technical difficulties
- 8:22: Brad Lowell at Harvard does not have UCP2, Invitrogen may have it looked up UCPs on invitrogen
- What expression vectors has the past iGEM teams used?
- We need E. coli expression vectors
- 8:32 Ahmad, ask them for the UCP
- 8:29: Indu wrote a nicely worded email.
- 8:31: Picking holins (there’s 3000!)
- There are some in the registry
- UC Berkeley from last year used Holin
- Ahmad will ask them for a couple Holins
- 8:49: CCDB – stops cell growth – can be another redundant mechanism
- It is in the registry
- 8:56: All of the parts so far are in the registry, we need a unique part to submit to the registry
- 9:01: We need to schedule a meeting with the panel for when we get back!
- June 17th
- Email the professors
- It would be nice to have Gary Wessel email them.
- 9:08: Michael write an email out to the professors inviting them
- Have the draft proofread by the group
- Have Wessel forward it to everybody
- 9:15: Somebody else – research a tight repressor!!
- 9:17: Indu will follow up phone call the lab for the tick protein
- Second idea to the histamine binding protein – have something that also binds IgE antibodies
- This provides redundancy – both binding current histamine and stopping further secretion of histamine
- Link them with glycine bridge
- 9:26: Probiotic system: natural bacteria that secretes protein into bloodstream
- We have getting it into the system from MIT yogurt
- We have intestinal implantation BactoKidney
- How do we get the bifunctional protein secreted?
- 9:44: There is a lady named Edith that Will will email – she can help us on probiotic drug transport across organs
- Ashley, Eli, Will, Minoo can research probiotic
- 9:45: Indu found a paper on protein that binds IGE antibody – she will put it on the google group
- Somebody needs to research IGE
- Eli will put up a paper about packaging
- Will is emailing about drug delivery systems
- 9:46: Any paper that may be useful to either project will be put on the google groups
- 9:56: Before 8pm PST – everyone go on the google group and build on the research that’s already been done.
- 10:00: Secretion: Eli, Will, Ashley
- OK everyone just decide what to research on the google group.
- 10:01: We are meeting on SUNDAY MAY 31 SAME TIME (Monday for Minoo)
- 10:02: Put up minutes.
- 10:16: bye everybody