Team:UNICAMP-Brazil/Notebooks/September 27
From 2009.igem.org
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====Digestion of pTet and Double Terminator==== | ====Digestion of pTet and Double Terminator==== | ||
- | *<p style=”text-align:justify;”>We | + | *<p style=”text-align:justify;”>We intend to ligate the parts which we are recovering right now (started yesterday) onto biobricks BBa_R0040 (pTet) and BBa_B0015 (double terminator), using it's own vector.</p> |
*<p style=”text-align:justify;”>Those biobricks were already recovered on August 10th.</p> | *<p style=”text-align:justify;”>Those biobricks were already recovered on August 10th.</p> | ||
- | *<p style=”text-align:justify;”>BBa_R0040 was digested with | + | *<p style=”text-align:justify;”>BBa_R0040 was digested with ''Spe''I and ''Pst''I, once we intend to add a fragment in front of it (downstream). As for BBa_B0015, the digestion was realized with ''EcoR''I and ''Xba''I restriction enzymes, once we need to insert a fragment behind it's part (upstream).</p> |
*<p style=”text-align:justify;”>Digestion lasted 3 hours.</p> | *<p style=”text-align:justify;”>Digestion lasted 3 hours.</p> | ||
Revision as of 03:19, 22 October 2009
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