Team:UNICAMP-Brazil/Notebooks/October 14
From 2009.igem.org
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====[https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/pGEMStrategy New Strategy: pGEM]==== | ====[https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/pGEMStrategy New Strategy: pGEM]==== | ||
- | * We did miniprep of 10 | + | * We did miniprep of 10 ''ADH1''+Lysozyme colonies, then we digested the Adh1+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector (YEP358). We used the ''Xba''I and ''Pst''I enzymes. 9 of 10 digestions worked! =) The expected fragment size is 2000bp. |
[[Image:20091021_confirmacao_adh1lys_results.png|300px|center]] | [[Image:20091021_confirmacao_adh1lys_results.png|300px|center]] |
Revision as of 03:45, 22 October 2009
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