Team:Warsaw/Calendar-Main/28 May 2009
From 2009.igem.org
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- | + | <h3>digest of constructs pKS/hly</h3> | |
- | + | <h4>Marcin</h4> | |
- | Restriction digest of isolated plasmids pKS/hly to confirm the correctness of the ligation | + | <br/> |
- | Methods: | + | <ul> |
- | + | <li>Task:</li> | |
- | + | </ul> | |
+ | <p>Restriction digest of isolated plasmids pKS/hly to confirm the correctness of the ligation</p> | ||
+ | <p>Methods:</p> | ||
+ | <ul> | ||
+ | <li>Composition of reaction mixture:1 μl purified plasmid solution, 0.5 μl HindIII (Fermentas), 2 μl Buffer Red (Fermentas), 15.5 μl MQ water</li> | ||
+ | <li>Digest</li> | ||
+ | </ul> | ||
+ | <br/> | ||
+ | <p>Program:</p> | ||
+ | <p>description of the program is <a href= "https://2009.igem.org/Team:Warsaw/Calendar-Main/13_July_2009">here</a></p> | ||
+ | <p>After digest samples were loaded into gel and electrophoretically separated</p> | ||
+ | <br/> | ||
+ | <p>Electrophoresis conditions:</p> | ||
+ | <p>voltage - 100V</p> | ||
+ | <p>time: 40 minutes</p> | ||
+ | <br/> | ||
+ | <img src="https://static.igem.org/mediawiki/2009/1/11/2009.05.28_-_trawienia_pKS%2Bhly_przyciety.jpg" height="30%" width="30%"> | ||
+ | <br/> | ||
+ | <strong>Comment:</strong> | ||
+ | <br/> | ||
+ | <p>Sample 1 and 2 have expected resctricion pattern.</p> | ||
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Revision as of 10:44, 18 July 2009
digest of constructs pKS/hly
Marcin
- Task:
Restriction digest of isolated plasmids pKS/hly to confirm the correctness of the ligation
Methods:
- Composition of reaction mixture:1 μl purified plasmid solution, 0.5 μl HindIII (Fermentas), 2 μl Buffer Red (Fermentas), 15.5 μl MQ water
- Digest
Program:
description of the program is here
After digest samples were loaded into gel and electrophoretically separated
Electrophoresis conditions:
voltage - 100V
time: 40 minutes
Comment:
Sample 1 and 2 have expected resctricion pattern.
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