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Team:Groningen/Notebook/8 August 2009
From 2009.igem.org
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==Wet== | ==Wet== | ||
| - | + | ===[http://openwetware.org/wiki/User:Wilfred_J._Poppinga/Notebook/Wilfreds_Project/2009/08/08 Miscellaneous]=== | |
| + | {| border="1" | ||
| + | |+'''Frans' plates''' | ||
| + | !<u>Colonies</u> | ||
| + | !<u>Name</u> | ||
| + | !<u>Plasmid</u> | ||
| + | !<u>Date of plating</u> | ||
| + | !<u>Antibiotics</u><BR> | ||
| + | |- | ||
| + | |<center>0</center> | ||
| + | |K3 pBad 2:3 | ||
| + | |pSB3K3 | ||
| + | |7august2009 | ||
| + | |50 μg/mL kan | ||
| + | |- | ||
| + | |<center>0</center> | ||
| + | |K3 pBad 2:3 | ||
| + | |pSB3K3 | ||
| + | |7august2009 | ||
| + | |50 μg/mL kan | ||
| + | |- | ||
| + | |<center>0</center> | ||
| + | |K3 pBad 2:4 | ||
| + | |pSB3K3 | ||
| + | |7august2009 | ||
| + | |50 μg/mL kan | ||
| + | |- | ||
| + | |<center>3</center> | ||
| + | |K3 pBad 2:4 | ||
| + | |pSB3K3 | ||
| + | |7august2009 | ||
| + | |50 μg/mL kan | ||
| + | |- | ||
| + | |<center>~150</center> | ||
| + | |AC3 p4od 1:3 | ||
| + | |pSB1AC3 | ||
| + | |7august2009 | ||
| + | |LB AMP | ||
| + | |- | ||
| + | |<center>~30</center> | ||
| + | |AC3 pBad 1:3 | ||
| + | |pSB1AC3 | ||
| + | |7august2009 | ||
| + | |LB AMP | ||
| + | |- | ||
| + | |<center>1</center> | ||
| + | |AC3 Lac 1:4 | ||
| + | |pSB1AC3 | ||
| + | |7august2009 | ||
| + | |LB AMP | ||
| + | |- | ||
| + | |<center>3</center> | ||
| + | |AC3 Lac 1:4 | ||
| + | |pSB1AC3 | ||
| + | |7august2009 | ||
| + | |LB AMP | ||
| + | |- | ||
| + | |} | ||
| + | After 5 h at 37 °C (and ON on the bench) Codes are written as they are read, not as they are interpreted. | ||
| + | Plates with no growth were put back on the bench. | ||
===GVP Cluster=== | ===GVP Cluster=== | ||
Latest revision as of 13:25, 8 August 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Wet
[http://openwetware.org/wiki/User:Wilfred_J._Poppinga/Notebook/Wilfreds_Project/2009/08/08 Miscellaneous]
| Colonies | Name | Plasmid | Date of plating | Antibiotics |
|---|---|---|---|---|
| K3 pBad 2:3 | pSB3K3 | 7august2009 | 50 μg/mL kan | |
| K3 pBad 2:3 | pSB3K3 | 7august2009 | 50 μg/mL kan | |
| K3 pBad 2:4 | pSB3K3 | 7august2009 | 50 μg/mL kan | |
| K3 pBad 2:4 | pSB3K3 | 7august2009 | 50 μg/mL kan | |
| AC3 p4od 1:3 | pSB1AC3 | 7august2009 | LB AMP | |
| AC3 pBad 1:3 | pSB1AC3 | 7august2009 | LB AMP | |
| AC3 Lac 1:4 | pSB1AC3 | 7august2009 | LB AMP | |
| AC3 Lac 1:4 | pSB1AC3 | 7august2009 | LB AMP |
After 5 h at 37 °C (and ON on the bench) Codes are written as they are read, not as they are interpreted. Plates with no growth were put back on the bench.
GVP Cluster
Transporters
Metal Accumulation
| Colonies | Name | Plasmid | Date of plating | Antibiotics |
|---|---|---|---|---|
| MymT Conc | pSB68(MymT) | 7august2009 | LB-Amp | |
| pSB1AC3-lowP | 7august2009 | LB-Cam150 | ||
| MymT | p4B68(MymT) | 7august2009 | LB-Amp | |
| pSB1AC3 | 7august2009 | LB-Cam150 | ||
| pSB1AC3-lowProm | 7august2009 | LB-Cam150 | ||
| (MymT Conc) | pGB68(MymT) conc. | 7august2009 | LB-Amp100 | |
| (MymT) | pGB68(MymT) | 7august2009 | LB-Amp | |
| pSB1AC3 | 7august2009 | LB-Cam150 |
Everything was written as [http://openwetware.org/wiki/User:Wilfred_J._Poppinga/Notebook/Wilfreds_Project/2009/08/08 I] read it, no guarantees.
Vectors
Dry
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