From 2009.igem.org
Wet
Frans' plates
Colonies
| Name
| Plasmid
| Date of plating
| Antibiotics
|
0
| K3 pBad 2:3
| pSB3K3
| 7august2009
| 50 μg/mL kan
|
0
| K3 pBad 2:3
| pSB3K3
| 7august2009
| 50 μg/mL kan
|
0
| K3 pBad 2:4
| pSB3K3
| 7august2009
| 50 μg/mL kan
|
3
| K3 pBad 2:4
| pSB3K3
| 7august2009
| 50 μg/mL kan
|
~150
| AC3 p4od 1:3
| pSB1AC3
| 7august2009
| LB AMP
|
~30
| AC3 pBad 1:3
| pSB1AC3
| 7august2009
| LB AMP
|
1
| AC3 Lac 1:4
| pSB1AC3
| 7august2009
| LB AMP
|
3
| AC3 Lac 1:4
| pSB1AC3
| 7august2009
| LB AMP
|
After 5 h at 37 °C (and ON on the bench) Codes are written as they are read, not as they are interpreted.
Plates with no growth were put back on the bench.
GVP Cluster
Transporters
Metal Accumulation
Nienke's plates MymT
Colonies
| Name
| Plasmid
| Date of plating
| Antibiotics
|
226
| MymT Conc
| pSB68(MymT)
| 7august2009
| LB-Amp
|
∞/10
| †
| pSB1AC3-lowP
| 7august2009
| LB-Cam150
|
12
| MymT
| p4B68(MymT)
| 7august2009
| LB-Amp
|
0
| –-
| pSB1AC3
| 7august2009
| LB-Cam150
|
∞
| (+)
| pSB1AC3-lowProm
| 7august2009
| LB-Cam150
|
>400
| (MymT Conc)
| pGB68(MymT) conc.
| 7august2009
| LB-Amp100
|
>400
| (MymT)
| pGB68(MymT)
| 7august2009
| LB-Amp
|
4
| –-
| pSB1AC3
| 7august2009
| LB-Cam150
|
Everything was written as I read it, no guarantees.
Vectors
Dry