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Team:UNICAMP-Brazil/Notebooks/August 11
From 2009.igem.org
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Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB media containg the appropriate antibiotic and let them grow O/N. | Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB media containg the appropriate antibiotic and let them grow O/N. | ||
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| + | ==Yeastguard== | ||
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| + | We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions. | ||
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| + | As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010. | ||
| + | So, planning to do the mini-prep tomorrow, we got the colonies from the plates (of the six biobricks) and put them to grow in liquid medium. | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 19:51, 14 August 2009
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