Team:UNICAMP-Brazil/Notebooks/July 24

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During the week from July 20th to 25th we designed all primers that we´re going to use in our ptoject. All the sequences are showed on July 25th notebook.
During the week from July 20th to 25th we designed all primers that we´re going to use in our ptoject. All the sequences are showed on July 25th notebook.
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==Coliguard: R Plasmid Mini-Preps==
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Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
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We performed mini-preps from R plasmid-containing cultures, according to protocol nº2 (see protocols section).
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As we did for F plasmid, we were also able to recover the R plasmid.
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'''Gabriel, Marcelo and Victor.'''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 21:26, 6 September 2009

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Primer design

During the week from July 20th to 25th we designed all primers that we´re going to use in our ptoject. All the sequences are showed on July 25th notebook.

Coliguard: R Plasmid Mini-Preps

Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process). We performed mini-preps from R plasmid-containing cultures, according to protocol nº2 (see protocols section). As we did for F plasmid, we were also able to recover the R plasmid.

Gabriel, Marcelo and Victor.





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