Team:UNICAMP-Brazil/Notebooks/July 24

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Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
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We performed mini-preps from R plasmid-containing cultures, according to protocol nº2 (see protocols section).
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We performed mini-preps from R plasmid-containing cultures, according to Protocol 2 (see protocols section).
As we did for F plasmid, we were also able to recover the R plasmid.
As we did for F plasmid, we were also able to recover the R plasmid.

Revision as of 16:49, 7 September 2009

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R Plasmid Mini-Preps

Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process). We performed mini-preps from R plasmid-containing cultures, according to Protocol 2 (see protocols section). As we did for F plasmid, we were also able to recover the R plasmid.

Gabriel, Marcelo and Victor.





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