Team:UNICAMP-Brazil/Notebooks/August 21

(Difference between revisions)

Revision as of 18:49, 7 September 2009

Today we perfomed miniprep from the RBS transformed E. coli. The fragment syze was correct and we decided to digest it without purifying. It worked.

After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol W.

Raíssa

@@ Line 1: / Line 1: @@
-== YeastGuard: Mini-prep, digestion and ligation of RBS ==
+{{:Team:UNICAMP-Brazil/inc_topo}}
+{{:Team:UNICAMP-Brazil/inc calendar}}
-- Today we perfomed mini-prep from the RBS transformed ''E. coli''. The fragment syze was correct,we decided to digest it without purifying and it worked.
+__NOTOC__
-- Afetr the RBS plasmid digestion we ligated it to CFP (the reporter gene) according to the protocol W.
+==''' YeastGuard '''==
+====Miniprep, digestion and ligation of RBS====
-'''Raíssa'''
+* Today we perfomed miniprep from the RBS transformed ''E. coli''. The fragment syze was correct and we decided to digest it without purifying. It worked.
+* After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol W.
+''Raíssa''
 {{:Team:UNICAMP-Brazil/inc_rodape}}