UQ-Australia/1 September 2009

From 2009.igem.org

(Difference between revisions)
(→Mercury Vacuum Project)
 
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=='''Mercury Vacuum Project'''==
=='''Mercury Vacuum Project'''==
 +
'''01/09/09  - Agarose Gel and Nanodrop'''<br/>
 +
* Miniprepped BBa_J63010 plasmids ran on 1% Agarose gel (TAE buffer, pH8.5) at 200V, 70mA for 1hour. Post-stain: Ethidium Bromide. Click [https://static.igem.org/mediawiki/2009/7/7e/UQ_-_Gel_1-09-09.tif HERE] for a picture of the gel. Lanes1,8 - empty, Lane2 - ladder, Lanes3-7 - Miniprepped DNA (colonies A-E)<br/>
 +
* Miniprepped BBa_J63010 plasmids (tubes A to E) analysed on nanodrop. (Data printout in IGEM_Mercury notebook)<br/>
 +
* New space created for BBa_J63010 tubes A to E: in "Fiona" freezer, UQ-Australia IGEM Team's ''Green'' box.<br/>
 +
* In the afternoon, digests of samples BBa_J63010 A-E (Standard 23) were run with EcoRI, AgeI, SpeI and PstI enzymes. Unfortunately NgoMIV enzymes were not in stock in the freezer so this digest could not be performed.
 +
* The Standard 21 plasmid BBa_I716101 was again digested with PstI. All samples were left in the 37 degrees water bath overnight.<br/>
<br/>
<br/>

Latest revision as of 05:39, 17 September 2009

Notebook

July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=UQ-Australia/1_July_2009&action=edit 1] [http://2009.igem.org/UQ-Australia/2_July_2009 2] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/14_July_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/15_July_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_July_2009&action=edit 16] [http://2009.igem.org/UQ-Australia/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/19_July_2009&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_July_2009&action=edit 20] [http://2009.igem.org/UQ-Australia/21_July_2009 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_July_2009&action=edit 22] [http://2009.igem.org/UQ-Australia/23_July_2009 23] [http://2009.igem.org/UQ-Australia/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/26_July_2009&action=edit 26]
[http://2009.igem.org/UQ-Australia/27_July_2009 27] [http://2009.igem.org/UQ-Australia/28_July_2009 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=UQ-Australia/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/2_August_2009&action=edit 2]
[http://2009.igem.org/UQ-Australia/3_August_2009 3] [http://2009.igem.org/UQ-Australia/4_August_2009 4] [http://2009.igem.org/UQ-Australia/5_August_2009 5] [http://2009.igem.org/UQ-Australia/6_August_2009 6] [http://2009.igem.org/UQ-Australia/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_August_2009&action=edit 9]
[http://2009.igem.org/UQ-Australia/10_August_2009 10] [http://2009.igem.org/UQ-Australia/11_August_2009 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_August_2009&action=edit 12] [http://2009.igem.org/UQ-Australia/13_August_2009 13] [http://2009.igem.org/UQ-Australia/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_August_2009&action=edit 16]
[http://2009.igem.org/UQ-Australia/17_August_2009 17] [http://2009.igem.org/UQ-Australia/18_August_2009 18] [http://2009.igem.org/UQ-Australia/19_August_2009 19] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/23_August_2009&action=edit 23]
[http://2009.igem.org/UQ-Australia/24_August_2009 24] [http://2009.igem.org/UQ-Australia/25_August_2009 25] [http://2009.igem.org/UQ-Australia/26_August_2009 26] [http://2009.igem.org/UQ-Australia/27_August_2009 27] [http://2009.igem.org/UQ-Australia/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/31_August_2009&action=edit 31]
September
MTWTFSS
  [http://2009.igem.org/UQ-Australia/1_September_2009 1] [http://2009.igem.org/UQ-Australia/2_September_2009 2] [http://2009.igem.org/UQ-Australia/3_September_2009 3] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/4_September_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/6_September_2009&action=edit 6]
[http://2009.igem.org/UQ-Australia/7_September_2009 7] [http://2009.igem.org/UQ-Australia/8_September_2009 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_September_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/10_September_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/11_September_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/13_September_2009&action=edit 13]
[http://2009.igem.org/UQ-Australia/14_September_2009 14] [http://2009.igem.org/UQ-Australia/15_September_2009 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_September_2009&action=edit 30]
October
MTWTFSS
      [http://2009.igem.org/wiki/index.php?title=UQ-Australia/1_October_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/2_October_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/4_October_2009&action=edit 4]
[http://2009.igem.org/UQ-Australia/5_October_2009 5] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/6_October_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/7_October_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/8_October_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/9_October_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/11_October_2009&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/12_October_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/13_October_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/14_October_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/15_October_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/16_October_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/17_October_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/18_October_2009&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/19_October_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/20_October_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/21_October_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=UQ-Australia/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/29_October_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/30_October_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=UQ-Australia/31_October_2009&action=edit 31]

Mercury Vacuum Project

01/09/09 - Agarose Gel and Nanodrop

  • Miniprepped BBa_J63010 plasmids ran on 1% Agarose gel (TAE buffer, pH8.5) at 200V, 70mA for 1hour. Post-stain: Ethidium Bromide. Click HERE for a picture of the gel. Lanes1,8 - empty, Lane2 - ladder, Lanes3-7 - Miniprepped DNA (colonies A-E)
  • Miniprepped BBa_J63010 plasmids (tubes A to E) analysed on nanodrop. (Data printout in IGEM_Mercury notebook)
  • New space created for BBa_J63010 tubes A to E: in "Fiona" freezer, UQ-Australia IGEM Team's Green box.
  • In the afternoon, digests of samples BBa_J63010 A-E (Standard 23) were run with EcoRI, AgeI, SpeI and PstI enzymes. Unfortunately NgoMIV enzymes were not in stock in the freezer so this digest could not be performed.
  • The Standard 21 plasmid BBa_I716101 was again digested with PstI. All samples were left in the 37 degrees water bath overnight.

Bioprecipitation Project
Retrieved from "http://2009.igem.org/UQ-Australia/1_September_2009"