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Team:Groningen/Notebook/18 September 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== '''Plates''' The ampicillin stocks showed signs of failure (growth on negative control, overgrowth of low concentration...) |
(→GVP Cluster) |
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'''Plates''' | '''Plates''' | ||
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{|cellpadding="2" cellspacing="1" border="4" | {|cellpadding="2" cellspacing="1" border="4" | ||
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|'''No. of Colonies''' | |'''No. of Colonies''' | ||
|'''Date''' | |'''Date''' | ||
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|- | |- | ||
|Positive (high) | |Positive (high) | ||
|J61002 (with J23101) | |J61002 (with J23101) | ||
|Ampicillin | |Ampicillin | ||
| - | |~ | + | |~1500 (red) |
| - | | | + | |17 sept. |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
|- | |- | ||
|Negative (high) | |Negative (high) | ||
|None | |None | ||
|None | |None | ||
| - | | | + | |8 (white/yellow) |
| - | | | + | |17 sept. |
| + | |- | ||
| + | |pMA-gvpL (low) | ||
| + | |pMA | ||
| + | |Ampicillin | ||
| + | |~150 | ||
| + | |17 sept. | ||
| + | |- | ||
| + | |pMA-gvpL (high) | ||
| + | |pMA | ||
| + | |Ampicillin | ||
| + | |~650 | ||
| + | |17 sept. | ||
| + | |- | ||
| + | |pMA-GlpF (low) | ||
| + | |pMA | ||
| + | |Ampicillin | ||
| + | |~125 | ||
| + | |17 sept. | ||
| + | |- | ||
| + | |pMA-gvpL (high) | ||
| + | |pMA | ||
| + | |Ampicillin | ||
| + | |~550 | ||
| + | |17 sept. | ||
| + | |- | ||
| + | |pNL29 (SJ)(low) | ||
| + | |pBlueScript_II_KS_(+) | ||
| + | |Ampicillin | ||
| + | |~100 | ||
| + | |17 sept. | ||
| + | |- | ||
| + | |pNL29 (high) | ||
| + | |pBlueScript_II_KS_(+) | ||
| + | |Ampicillin | ||
| + | |~250 | ||
| + | |17 sept. | ||
| + | |- | ||
| + | |pArsR-GVP (Glycerol Stock) | ||
| + | |pSB2K3 | ||
| + | |Kanamycin | ||
| + | |Single Colonies | ||
| + | |17 sept. | ||
| + | |- | ||
| + | |pArsR-GVP (Saline Test) | ||
| + | |pSB2K3 | ||
| + | |Kanamycin | ||
| + | |Plate Full-Grown | ||
| + | |17 sept. | ||
| + | |- | ||
| + | |pZntR-GVP (Saline Test) | ||
| + | |pSB2K3 | ||
| + | |Kanamycin | ||
| + | |Plate Full-Grown | ||
| + | |17 sept. | ||
| + | |- | ||
| + | |pCueO-GVP (Saline Test) | ||
| + | |pSB2K3 | ||
| + | |Kanamycin | ||
| + | |Plate Full-Grown | ||
| + | |17 sept. | ||
|} | |} | ||
| - | :→ The plates showed the expected amount of colonies, and also red in the case of the positive plates. The | + | :→ The plates showed the expected amount of colonies, and also red in the case of the positive plates. The eight colonies on the negative plate can be taken into acount when plating transformed cells. |
| - | :→ The | + | :→ The plate with stripes of pArsR-GVP (pSB2K3) preculture showed growth and some single colonies. |
| - | :→ All | + | :→ All plates were stored in the fridge for further use on monday. |
'''Cultures''' | '''Cultures''' | ||
| - | The overnight cultures with LB-amp<sub>100</sub> medium of colonies E.coli TOP10 with | + | The overnight cultures with LB-amp<sub>100</sub> medium of colonies E.coli TOP10 with pNL29 in pBlueScript_II_KS_(+) showed no growth. |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
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| + | :→ The plate used for culture inoculation was very (more than a month) old, and was probably no good anymore. The new transformed E.coli TOP10 with original pNL29 plasmid will replace all excisting stocks/plates. | ||
===Transporters=== | ===Transporters=== | ||
Revision as of 11:58, 18 September 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
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Wet
GVP Cluster
Plates
| Name | Plasmid Used | Antibiotics on Plasmid | No. of Colonies | Date |
| Positive (high) | J61002 (with J23101) | Ampicillin | ~1500 (red) | 17 sept. |
| Negative (high) | None | None | 8 (white/yellow) | 17 sept. |
| pMA-gvpL (low) | pMA | Ampicillin | ~150 | 17 sept. |
| pMA-gvpL (high) | pMA | Ampicillin | ~650 | 17 sept. |
| pMA-GlpF (low) | pMA | Ampicillin | ~125 | 17 sept. |
| pMA-gvpL (high) | pMA | Ampicillin | ~550 | 17 sept. |
| pNL29 (SJ)(low) | pBlueScript_II_KS_(+) | Ampicillin | ~100 | 17 sept. |
| pNL29 (high) | pBlueScript_II_KS_(+) | Ampicillin | ~250 | 17 sept. |
| pArsR-GVP (Glycerol Stock) | pSB2K3 | Kanamycin | Single Colonies | 17 sept. |
| pArsR-GVP (Saline Test) | pSB2K3 | Kanamycin | Plate Full-Grown | 17 sept. |
| pZntR-GVP (Saline Test) | pSB2K3 | Kanamycin | Plate Full-Grown | 17 sept. |
| pCueO-GVP (Saline Test) | pSB2K3 | Kanamycin | Plate Full-Grown | 17 sept. |
- → The plates showed the expected amount of colonies, and also red in the case of the positive plates. The eight colonies on the negative plate can be taken into acount when plating transformed cells.
- → The plate with stripes of pArsR-GVP (pSB2K3) preculture showed growth and some single colonies.
- → All plates were stored in the fridge for further use on monday.
Cultures
The overnight cultures with LB-amp100 medium of colonies E.coli TOP10 with pNL29 in pBlueScript_II_KS_(+) showed no growth.
- → The plate used for culture inoculation was very (more than a month) old, and was probably no good anymore. The new transformed E.coli TOP10 with original pNL29 plasmid will replace all excisting stocks/plates.
Transporters
Metal Accumulation
Vectors
Dry
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