Team:Kyoto/CiC/Notebook

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0907-0911: constructing HIV-TAT-(LALAAAA)3-generator
0914-0918: constructing HIV-TAT-(LALAAAA)3-generator
0921-0925: constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002: constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009: constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016: Observation
1019-1023: Observation

Monday

To Do on 24 July

Miniprep (3-O-2, 3-A-4)
Restriction Enzyme Digestion
Electrophoresis

Results

Miniprep

3-O-2, 3-A-4 was minipreped.

Digestion

Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.

Sample list
NO.	Sample Name	Enzyme
1	3-O-2	Eco R1, PST1
2	3-A-4	Eco R1, PST1
3	3-O-2	Eco R1
4	3-A-4	Eco R1

Electrophoresis

Very clear bands were found. (See the gel image in right!)
The bands are listed below.

Parts and Plasmid length
	3-O-2	3-A-4
Parts length / bp	2837	487
Plasmid length / bp	3266	3266

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