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Team:Kyoto/CiC/Notebook
From 2009.igem.org
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{{:Team:Kyoto/CiC/Notebook_tpl}} | {{:Team:Kyoto/CiC/Notebook_tpl}} | ||
| + | {{:Team:Kyoto/Pad_start_dates}} | ||
| + | ===Monday=== | ||
| + | ====To Do on 24 July==== | ||
| + | *Miniprep (3-O-2, 3-A-4) | ||
| + | *Restriction Enzyme Digestion | ||
| + | *Electrophoresis | ||
| + | ====Results==== | ||
| + | =====Miniprep===== | ||
| + | *3-O-2, 3-A-4 was minipreped. | ||
| - | === | + | =====Digestion===== |
| + | *Digested with EcoR1 / EcoR1 and Pst1 and run on a gel. | ||
| + | |||
| + | {| border="1" style="text-align:center;width:50%" | ||
| + | |+Sample list | ||
| + | !NO.!!Sample Name!!Enzyme | ||
| + | |-align="center" | ||
| + | |1||3-O-2||Eco R1, PST1 | ||
| + | |-align="center" | ||
| + | |2||3-A-4||Eco R1, PST1 | ||
| + | |-align="center" | ||
| + | |3||3-O-2||Eco R1 | ||
| + | |-align="center" | ||
| + | |4||3-A-4||Eco R1 | ||
| + | |} | ||
| + | |||
| + | =====Electrophoresis===== | ||
| + | [[Image:Kyoto_e_090724_1.png|250px]] | ||
| + | * Very clear bands were found. (See the gel image in right!) | ||
| + | * The bands are listed below. | ||
| + | |||
| + | {| border="1" style="text-align:center;width:50%" | ||
| + | |+Parts and Plasmid length | ||
| + | !!!3-O-2!!3-A-4 | ||
| + | |- | ||
| + | |Parts length / bp||2837||487 | ||
| + | |- | ||
| + | |Plasmid length / bp||3266||3266 | ||
| + | |} | ||
| + | {{:Team:Kyoto/Pad_between_dates}} | ||
| + | ===Tuseday=== | ||
====To Do on 24 July==== | ====To Do on 24 July==== | ||
*Miniprep (3-O-2, 3-A-4) | *Miniprep (3-O-2, 3-A-4) | ||
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|Plasmid length / bp||3266||3266 | |Plasmid length / bp||3266||3266 | ||
|} | |} | ||
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Revision as of 18:17, 25 September 2009
- Home
- Cells in Cells
- Notebook
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
Monday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
Tuseday
To Do on 24 July
- Miniprep (3-O-2, 3-A-4)
- Restriction Enzyme Digestion
- Electrophoresis
Results
Miniprep
- 3-O-2, 3-A-4 was minipreped.
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
