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Team:UNICAMP-Brazil/Notebooks/August 15
From 2009.igem.org
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* Today we started the construction of our first biobrick. After quantificate the miniprep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter. | * Today we started the construction of our first biobrick. After quantificate the miniprep's products (from 11/Aug) we performed the digestion (protocol Y) of plasmids containing the RBS and the CFP reporter. | ||
| - | * After the digestion we isolated the target bands from the agarose gel, according to the protocol | + | * After the digestion we isolated the target bands from the agarose gel, according to the protocol 7. |
* The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today. | * The CFP digested band was correctly purified from the gel, unfortunatly we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate this two parts today. | ||
Revision as of 18:42, 26 September 2009
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