Team:UNICAMP-Brazil/Notebooks/September 13

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(→Lysozyme (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter))
(→Lysozyme (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter))
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* We performed miniprep to get purified these parts (Protocol 2).  
* We performed miniprep to get purified these parts (Protocol 2).  
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* We then digest the plasmids obtained by the miniprep (Protocol x).  
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* We then digest the plasmids obtained by the miniprep with ''Xba''I (Protocol x).  
* The electrophoresis gel (down) showed nonspecific bands of al the parts digested.  
* The electrophoresis gel (down) showed nonspecific bands of al the parts digested.  

Revision as of 20:37, 26 September 2009

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ColiGuard

finO+pSB1A3

  • After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
  • We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.

finP+pSB1A3

  • We followed the same produce for the finP+pSB1A3 ligation.

Marcelo

YeastGuard

Lysozyme (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter)

  • We performed miniprep to get purified these parts (Protocol 2).
  • We then digest the plasmids obtained by the miniprep with XbaI (Protocol x).
  • The electrophoresis gel (down) showed nonspecific bands of al the parts digested.
‎

Wesley and Gleidson




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