Team:UNICAMP-Brazil/Notebooks/September 13

(Difference between revisions)

Revision as of 20:43, 26 September 2009

After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.

Marcelo

We performed miniprep to get purified the plasmids containing these parts. (Protocol 2).
We then digest the plasmids obtained by the miniprep with XbaI to confirm the correct ligation of the parts following the biobrick format (Protocolo x). However, the electrophoresis gel (down) showed no success in the ligations.

Wesley and Gleidson

@@ Line 20: / Line 20: @@
 ====Lysozyme (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter)====
-* We performed miniprep to get purified these parts (Protocol 2).
+* We performed miniprep to get purified the plasmids containing these parts. (Protocol 2).
-* We then digest the plasmids obtained by the miniprep with ''Xba''I (Protocol x).
+* We then digest the plasmids obtained by the miniprep with ''XbaI'' to confirm the correct ligation of the parts following the biobrick format (Protocolo x). However, the electrophoresis gel (down) showed no success in the ligations.
-* The electrophoresis gel (down) showed nonspecific bands of al the parts digested.
 [[Image:MInipreps e digestões Wesley.jpg |center|‎]]