Team:UNICAMP-Brazil/Notebooks/September 13

(Difference between revisions)

Revision as of 21:25, 26 September 2009

After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.

Marcelo

6 colonies of each new biobrick were chosen of the plates made yesterday to grow in liquid LB.
Then, we performed miniprep to get purified our new biobricks(Protocol 2).
We then digest the plasmids obtained by the miniprep with XbaI to confirm the correct ligation of the parts following the biobrick format (Protocolo x). However, the electrophoresis gel (down) showed a unexpected pattern of bands that can't confirm correctly ligations of our biobricks. =(

Wesley and Gleidson

@@ Line 20: / Line 20: @@
 ====New biobrick in biobrick format====
+* 6 colonies of each new biobrick were chosen of the plates made yesterday to grow in liquid LB.
-* We performed miniprep to get purified the plasmids containing these parts. (Protocol 2).
+* Then, we performed miniprep to get purified our new biobricks(Protocol 2).
-* We then digest the plasmids obtained by the miniprep with ''Xba''I to confirm the correct ligation of the parts following the biobrick format (Protocolo x). However, the electrophoresis gel (down) showed no success in the ligations.
+* We then digest the plasmids obtained by the miniprep with ''Xba''I to confirm the correct ligation of the parts following the biobrick format (Protocolo x). However, the electrophoresis gel (down) showed a unexpected pattern of bands that can't confirm correctly ligations of our biobricks. =(
 [[Image:MInipreps e digestões Wesley.jpg |center|‎]]