Team:UNICAMP-Brazil/Notebooks/September 22

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==''' ColiGuard '''==
====Electroelution verification====
====Electroelution verification====

Revision as of 22:17, 26 September 2009

Contents

ColiGuard

Topo l2.gif topo_r_igem.gif
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Back to Calendar

Topo l2.gif topo_r_igem.gif
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Back to Calendar

ColiGuard

Electroelution verification

  • The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
  • Now that the F plasmid was obtained, the next step is its recircularization.

Gabriel