Team:IBB Pune/Protocols

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Maintenance of microbial cultures

Standard cultures use in out project are

1) E.coli DH5a 2) E.coli JM101 3) Acinetobacter baylyi BD413 4) Streptococcus pneumoniae R6

Glycerol stocks of all the strains are maintained at -80oC. Strains are maintained on working plates for daily use, subcultured every week. Working plates use are Luria Bertani Agar for E.coli and Sterptococcusstrains and CLED (Cysteine Lactose Electrolyte Deficient) Agar for Acinetobactersp.

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+== Maintenance of microbial cultures ==
+ Standard cultures use in out project are
+) ''E.coli DH5a''
+) ''E.coli JM101''
+) ''Acinetobacter baylyi BD413''
+) ''Streptococcus pneumoniae R6''
+Glycerol stocks of all the strains are maintained at -80oC.
+Strains are maintained on working plates for daily use, subcultured every week. Working plates use are Luria Bertani Agar for ''E.coli'' and ''Sterptococcus''strains and CLED (Cysteine Lactose Electrolyte Deficient) Agar for ''Acinetobacter''sp.