Team:UNICAMP-Brazil/Notebooks/September 22

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''Gabriel''
''Gabriel''
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====finO and finP - Still Trying to Confirm our Biobricks====
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* We innoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP media, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.
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''Marcelo''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 18:02, 27 September 2009

Contents

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ColiGuard

Electroelution verification

  • The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
  • Now that the F plasmid was obtained, the next step is its recircularization.

Gabriel

finO and finP - Still Trying to Confirm our Biobricks

  • We innoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP media, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.


Marcelo





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