Team:UNICAMP-Brazil/Notebooks/September 25
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* Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D | * Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D | ||
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+ | ====finO and finP - Still Trying to Confirm our Biobricks==== | ||
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+ | * We ran an agarose gel of yesterday's PCRs product. | ||
+ | * We couldn't obtain even a single amplified fragment! =( | ||
+ | * Why can the transformed cells grew in the media (that means they actually have the AMP resistence), but they haven't our inserts into it's plasmids? | ||
+ | * Our advisors suggested us that, since we digested our plasmid vector with Xba and SpeI (X sticky ends can come together with S sticky ends), it recircularized without the introduction of our inserts. | ||
+ | * Therefore, we must perform the dephosphorylation of our digested vector. That may prevent it from recircularizing without the insert introduction. | ||
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+ | ''Marcelo'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Revision as of 18:46, 27 September 2009
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