Team:UNICAMP-Brazil/Notebooks/September 25

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* Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D
* Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D
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====finO and finP - Still Trying to Confirm our Biobricks====
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* We ran an agarose gel of yesterday's PCRs product.
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* We couldn't obtain even a single amplified fragment! =(
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* Why can the transformed cells grew in the media (that means they actually have the AMP resistence), but they haven't our inserts into it's plasmids?
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* Our advisors suggested us that, since we digested our plasmid vector with Xba and SpeI (X sticky ends can come together with S sticky ends), it recircularized without the introduction of our inserts.
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* Therefore, we must perform the dephosphorylation of our digested vector. That may prevent it from recircularizing without the insert introduction.
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''Marcelo''
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{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 18:46, 27 September 2009

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Contents

ColiGuard

E. coli F+ culture

  • About 10 white colonies appeared at the culture plate, indicating that the cells incorporated the F plasmid. The plate was lacked and stored at 4°C.

Gabriel

Getting the visa

  • Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D

finO and finP - Still Trying to Confirm our Biobricks

  • We ran an agarose gel of yesterday's PCRs product.
  • We couldn't obtain even a single amplified fragment! =(
  • Why can the transformed cells grew in the media (that means they actually have the AMP resistence), but they haven't our inserts into it's plasmids?
  • Our advisors suggested us that, since we digested our plasmid vector with Xba and SpeI (X sticky ends can come together with S sticky ends), it recircularized without the introduction of our inserts.
  • Therefore, we must perform the dephosphorylation of our digested vector. That may prevent it from recircularizing without the insert introduction.


Marcelo




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