Team:UNICAMP-Brazil/Notebooks/September 26
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====Recovering More Biobricks==== | ====Recovering More Biobricks==== | ||
- | * We started our "new strategy" (related to modifying Paris 2007 team's biobricks) by recovering some biobricks that had now became useful for our project. We ressuspended biobricks BBa_E0840 (GFP device), BBa_I718017 (lox71), BBa_J61000 (chloramphenicol resistance cassette) and BBa_I718016 (lox66), and then transfomed them into electrocompetent E. coli cells, strain DH10B, according to protocol | + | * We started our "new strategy" (related to modifying Paris 2007 team's biobricks) by recovering some biobricks that had now became useful for our project. We ressuspended biobricks BBa_E0840 (GFP device), BBa_I718017 (lox71), BBa_J61000 (chloramphenicol resistance cassette) and BBa_I718016 (lox66), and then transfomed them into electrocompetent E. coli cells, strain DH10B, according to protocol 3 (see Protocols section). |
* The transformed cells were plated in LB-AMP media, and were grown for an O/N period. | * The transformed cells were plated in LB-AMP media, and were grown for an O/N period. | ||
Revision as of 23:42, 29 September 2009
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