Team:UNICAMP-Brazil/Notebooks/September 27

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''Marcelo''
''Marcelo''
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====Digestion of pTet and Double Terminator====
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* We pretend to ligate the parts which we are recovering right now (started yesterday) onto biobricks BBa_R0040 (pTet) and BBa_B0015 (double terminator), using it's own vector.
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* Those biobricks were already recovered on August 10th.
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* BBa_R0040 was digested with SpeI and PstI, once we intend to add a fragment in front of it (downstream). As for BBa_B0010, the digestion was realized with EcoRI and Xba restriction enzymes, once we need to insert a fragment behind it's part (upstream).
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* Digestion lasted 3 hours.
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''Marcelo''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 00:10, 30 September 2009

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YeastGuard

Dephosphorylation - CIAP test

  • The E.coli didn´t grow in any of the plates. =[ We think that we didn´t plate an enough volume of bacteria. So we decided to replate a larger volume. We hope to find colonies tomorrow!


Raíssa and Taís

ColiGuard

Inoculation of Yesterday's Ressuspended and Transformed Biobricks

  • We selected 4 colonies from each plate containing one of yesterday's transformed biobricks, and inoculated them in liquid LB-AMP media.
  • Those inocula were grew for an O/N period at 37ºC (250 rpm).

Marcelo


Digestion of pTet and Double Terminator

  • We pretend to ligate the parts which we are recovering right now (started yesterday) onto biobricks BBa_R0040 (pTet) and BBa_B0015 (double terminator), using it's own vector.
  • Those biobricks were already recovered on August 10th.
  • BBa_R0040 was digested with SpeI and PstI, once we intend to add a fragment in front of it (downstream). As for BBa_B0010, the digestion was realized with EcoRI and Xba restriction enzymes, once we need to insert a fragment behind it's part (upstream).
  • Digestion lasted 3 hours.

Marcelo





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