Team:UNICAMP-Brazil/Notebooks/August 28

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YeastGuard

RBS+CFP

We did the permanent colonies.

Taís

Kluyveromices lactis genes

The PCR for the K. lactis genes worked very well! =] We got specific bands for the 3 sequences.

GEL PCR

Taís

Terminator biobrick

Colony PCR

The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics.

We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denatured this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol).

Preparing for Miniprep

We also inoculated ten colonies transformed with the terminator biobrick to do the miniprep tomorrow.

Wesley

@@ Line 24: / Line 24: @@
 The colony PCR was made to confirm the presence of terminator transformants, because  the antibiotic resistence is not working to any antibiotics.
-We plated the the cells in LB, and picked some colonies with a toothpick, inoculate on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denature this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol).
+We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denatured this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol).
 * Preparing for Miniprep
-We also innoculated ten colonies transformed with the terminator biobrick to do the miniprep tomorrow.
+We also inoculated ten colonies transformed with the terminator biobrick to do the miniprep tomorrow.
 ''Wesley''
 {{:Team:UNICAMP-Brazil/inc_rodape}}