Team:UNICAMP-Brazil/Notebooks/September 25
From 2009.igem.org
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* Our advisors suggested us that, since we digested our plasmid vector with Xba and SpeI (X sticky ends can come together with S sticky ends), it recircularized without the introduction of our inserts. | * Our advisors suggested us that, since we digested our plasmid vector with Xba and SpeI (X sticky ends can come together with S sticky ends), it recircularized without the introduction of our inserts. | ||
* Therefore, we must perform the dephosphorylation of our digested vector. That may prevent it from recircularizing without the insert introduction. | * Therefore, we must perform the dephosphorylation of our digested vector. That may prevent it from recircularizing without the insert introduction. | ||
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''Marcelo'' | ''Marcelo'' | ||
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==''' YeastGuard '''== | ==''' YeastGuard '''== |
Revision as of 14:24, 2 October 2009
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