Team:UNICAMP-Brazil/Notebooks/September 30

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*<p style=”text-align:justify;”>This morning we found some ''E. coli'' colonies that were transformed yesterday! We performed PCR with 10 colonies from each biobrick (pJEN1, pDLD and Lysozyme) with the vector's primers (VR and VF).   
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*This morning we found some ''E. coli'' colonies that were transformed yesterday! We performed PCR with 10 colonies from each biobrick (pJEN1, pDLD and Lysozyme) with the vector's primers (VR and VF).   
- The figure shows that unfortunately none of the colonies tested had the insert into the vector. The size of the amplicons correspond to the vector's fragment without any insert (+/- 1400bp).  
- The figure shows that unfortunately none of the colonies tested had the insert into the vector. The size of the amplicons correspond to the vector's fragment without any insert (+/- 1400bp).  

Revision as of 21:04, 3 October 2009

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YeastGuard

New biobricks - screening

  • This morning we found some E. coli colonies that were transformed yesterday! We performed PCR with 10 colonies from each biobrick (pJEN1, pDLD and Lysozyme) with the vector's primers (VR and VF).
- The figure shows that unfortunately none of the colonies tested had the insert into the vector. The size of the amplicons correspond to the vector's fragment without any insert (+/- 1400bp).
UNICAMP 20090930 GEL1.png

- We also decided to try to do a miniprep using the rest of the transformed E. coli that was not plated in LB+AMP media. So today we made the inoculum.

Raíssa and Taís





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