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Team:UNICAMP-Brazil/Notebooks/September 25
From 2009.igem.org
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====''E. coli'' F+ culture==== | ====''E. coli'' F+ culture==== | ||
| - | *About 10 white colonies appeared at the culture plate, indicating that the cells incorporated the F plasmid. The plate was lacked and stored at 4°C. | + | *<p style=”text-align:justify;”>About 10 white colonies appeared at the culture plate, indicating that the cells incorporated the F plasmid. The plate was lacked and stored at 4°C.</p> |
''Gabriel'' | ''Gabriel'' | ||
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====Getting the visa==== | ====Getting the visa==== | ||
| - | * Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D | + | *<p style=”text-align:justify;”>Today was a happy day to five of us! We went to the U.S. Embassy in Sao Paulo and we got our visas! :D</p> |
====finO and finP - Still Trying to Confirm our Biobricks==== | ====finO and finP - Still Trying to Confirm our Biobricks==== | ||
| - | * We ran an agarose gel of yesterday's PCRs product. | + | *<p style=”text-align:justify;”>We ran an agarose gel of yesterday's PCRs product.</p> |
| - | * We couldn't obtain even a single amplified fragment! =( | + | *<p style=”text-align:justify;”>We couldn't obtain even a single amplified fragment! =(</p> |
| - | * Why can the transformed cells grew in the media (that means they actually have the AMP resistence), but they haven't our inserts into it's plasmids? | + | *<p style=”text-align:justify;”>Why can the transformed cells grew in the media (that means they actually have the AMP resistence), but they haven't our inserts into it's plasmids?</p> |
| - | * Our advisors suggested us that, since we digested our plasmid vector with Xba and | + | *<p style=”text-align:justify;”>Our advisors suggested us that, since we digested our plasmid vector with ''Xba''I and ''Spe''I (X sticky ends can come together with S sticky ends), it recircularized without the introduction of our inserts.</p> |
| - | * Therefore, we must perform the dephosphorylation of our digested vector. That may prevent it from recircularizing without the insert introduction. | + | *<p style=”text-align:justify;”>Therefore, we must perform the dephosphorylation of our digested vector. That may prevent it from recircularizing without the insert introduction.</p> |
''Marcelo'' | ''Marcelo'' | ||
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====Biofusion vector - Electroelution==== | ====Biofusion vector - Electroelution==== | ||
| - | Considering that we need more vector to perform the ligation reactions, we electroeluted more vector from the ADH1 biobrick previously digested with | + | *<p style=”text-align:justify;”>Considering that we need more vector to perform the ligation reactions, we electroeluted more vector from the ADH1 biobrick previously digested with ''Xba''1 and ''Spe''1 ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroelution Protocol 12]).</p> |
''Taís'' | ''Taís'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Revision as of 21:29, 3 October 2009
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