Team:UNICAMP-Brazil/Notebooks/September 22

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ColiGuard

Electroelution verification

The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
Now that the F plasmid was obtained, the next step is its recircularization.

Gabriel

finO and finP - Still Trying to Confirm our Biobricks

We inoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP medium, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.

Marcelo

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 ====Electroelution verification====
-*The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
+*<p style=”text-align:justify;”>The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!</p>
-*Now that the F plasmid was obtained, the next step is its recircularization.
+*<p style=”text-align:justify;”>Now that the F plasmid was obtained, the next step is its recircularization.</p>
 ''Gabriel''
@@ Line 17: / Line 15: @@
 ====finO and finP - Still Trying to Confirm our Biobricks====
-* We inoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP media, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.
+*<p style=”text-align:justify;”>We inoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP medium, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.</p>
 ''Marcelo''