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Team:UNICAMP-Brazil/Notebooks/September 12
From 2009.igem.org
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====finO+pSB1A3 ligation==== | ====finO+pSB1A3 ligation==== | ||
| - | * Since all of our fragments are correctly digested, we started today the ligation of both finO and finP sequences to the vector pSB1A3. Each sequence was ligated singly to the vector and, only after each one is confirmed, we'll join then both to a single construction. | + | *<p style=”text-align:justify;”>Since all of our fragments are correctly digested, we started today the ligation of both finO and finP sequences to the vector pSB1A3. Each sequence was ligated singly to the vector and, only after each one is confirmed, we'll join then both to a single construction.</p> |
| - | * We performed the ligation finO+vector pSB1A3 for an O/N period, according to protocol Y (see Protocols section). | + | *<p style=”text-align:justify;”>We performed the ligation finO+vector pSB1A3 for an O/N period, according to protocol Y (see Protocols section).</p> |
====finP+pSB1A3 ligation==== | ====finP+pSB1A3 ligation==== | ||
| - | * As we did for finO sequence, we also ligated finP to the vector pSB1A3 according to the same protocol. The ligation also lasted O/N period. | + | *<p style=”text-align:justify;”>As we did for finO sequence, we also ligated finP to the vector pSB1A3 according to the same protocol. The ligation also lasted O/N period.</p> |
''Marcelo'' | ''Marcelo'' | ||
Revision as of 22:03, 3 October 2009
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