Team:UNICAMP-Brazil/Notebooks/September 4

(Difference between revisions)

Revision as of 22:51, 3 October 2009

Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4.

Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction. We ressuspended the biobrick of Cre-Recombinase: BBa_J61047 and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.

Víctor

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 ==== Cre-Recombinase without ATG ====
-*<p style=”text-align:justify;”>Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction.  We ressuspended the biobrick of Cre-Recombinase: BBa_J61047  and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.</p>
+*<p style=”text-align:justify;”>Today we started the construction of Cre-Recombinase without ATG, in order to made a new biobrick involving this construction. We ressuspended the biobrick of Cre-Recombinase: BBa_J61047  and transformed in E. coli DH10B eletrocompetent cells. We then plated on LB Amp plate, according to information from registry page.</p>
 ''Víctor''
 {{:Team:UNICAMP-Brazil/inc_rodape}}