Team:Newcastle/Labwork/18 September 2009

From 2009.igem.org

(Difference between revisions)
(New page: {{:Team:Newcastle/CSS}} {{:Team:Newcastle/Header}} {{:Team:Newcastle/Left}} __NOTOC__ =Lab Work - 18/09/09= =Stochastic switch team= Today we did midipreps of the ara transformation and d...)
Line 3: Line 3:
{{:Team:Newcastle/Left}}
{{:Team:Newcastle/Left}}
__NOTOC__
__NOTOC__
-
=Lab Work - 18/09/09=
+
[[Image:Team Newcastle 2009 iGEM ProbationaryP-Sign.PNG|50px|right]]
 +
=Formal Lab Session - 18th September 2009=
=Stochastic switch team=
=Stochastic switch team=

Revision as of 16:16, 6 October 2009

Team Newcastle 2009 iGEM ProbationaryP-Sign.PNG

Formal Lab Session - 18th September 2009

Stochastic switch team

Today we did midipreps of the ara transformation and digested 10ul to run on a gel (EcoRI + PstI) Two bands could be seen one for the backbone and one correctly sied for the insert (~200bp) so this midiprep can be taken forward in order to do the ara-sspb double clone.

Today we also made up some fresh DNA ladder as we have had problems with degradation.

  • 40ul of 500ug/ml promega lambda HindIII digest (20ug in total)
  • 280ul H2O
  • 80ul loading dye

=400ul which was aliquoted into 4 tubes; 3 put in the freezer 1 in the fridge.



News

Events

Social Net

  • Newcastle iGEM Twitter
  • [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
  • [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
Retrieved from "http://2009.igem.org/Team:Newcastle/Labwork/18_September_2009"