Team:UNICAMP-Brazil/Notebooks/October 7

From 2009.igem.org

(Difference between revisions)
(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard'''== ====New biobricks - New strategy ==== - We performed PCR of 7 colonies of each c...)
(New biobricks - New strategy)
Line 6: Line 6:
==''' YeastGuard'''==
==''' YeastGuard'''==
-
====New biobricks - New strategy ====
+
====New biobricks - New strategy====
 +
 
- We performed PCR of 7 colonies of each construction (Jen1_orf and Jen1_promoter) inoculated yesterday.  
- We performed PCR of 7 colonies of each construction (Jen1_orf and Jen1_promoter) inoculated yesterday.  
We found 4 positive colonies for Jen1_promoter! =)
We found 4 positive colonies for Jen1_promoter! =)
-
 
-
- Unfortunately no Jen1_orf construction was found in colonies selected. To screen a bigger number of colonies we inoculated 10 more colonies in liquid media.
 
GEL
GEL
-
''Raíssa''
+
- Unfortunately no Jen1_orf construction was found in colonies selected but we continued screenig! To find positive colonies we screened 10 more colonies and made a backup inoculum in case we find right ones. Tomorrow we'll have the answer.
 +
''Raíssa''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 15:17, 8 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

New biobricks - New strategy

- We performed PCR of 7 colonies of each construction (Jen1_orf and Jen1_promoter) inoculated yesterday. We found 4 positive colonies for Jen1_promoter! =)

GEL

- Unfortunately no Jen1_orf construction was found in colonies selected but we continued screenig! To find positive colonies we screened 10 more colonies and made a backup inoculum in case we find right ones. Tomorrow we'll have the answer.

Raíssa