Team:UNICAMP-Brazil/Notebooks/October 5

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' ColiGuard '''== ====finOP and Cre-Recombinase with pGEM strategy==== * Today we performed minip...)
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Revision as of 20:26, 10 October 2009

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ColiGuard

finOP and Cre-Recombinase with pGEM strategy

  • Today we performed minipreps from yesterday's inoculated cultures, according to protocol 2.
  • We expect to recover pGEM plasmids finally containing singly our inserts for finO, finP and Cre-Recombinase w/o ATG.
  • As both sides of the inserts contains the A stick end, it's possible that we will find in some samples unwanted ligations (it could be ligate in an upside down position). Therefore, we will also need to perform PCRs to confirm that our inserts indeed are in the correct frame position.

Marcelo and Victor




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