Team:Cambridge/Project/Pigments
From 2009.igem.org
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Pigments
- Colour: Though E. coli does not naturally produce pigment, several other bacterial species secrete pigmented antibiotics. We have used pigment-producing operons from other bacterial species and transformed them into E. coli. In particular, we have devoted our summer to 3 different pigment systems:
- [Team:Cambridge/Project/CA01 Carotenoids]: The enzymes required for carotenoid production originally come from Pantoea ananatis, and were available in the registry. We used them to produce orange and red.
- [Team:Cambridge/Project/ME01 Melanin]: The tyrosinase required for melanin production originally comes from Rhizobium etli and produces brown.
- [Team:Cambridge/Project/VI01 Violacein]: The enzymes required for voilacein production originally come from Chromobacterium violacein. The operon can be manipulated to produce voilet, green, and blue.