Team:UNICAMP-Brazil/Notebooks/August 5

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Preparation of electrocompetent E. coli

Today we prepared electrocompetent E. coli to use in our transformations according to protocol 4 (see protocol section).

Marcelo and Luige

Yeast DNA extraction

Today we performed DNA extration from yeast Kluyveromyces lactis according to protocol 5(see protocol section). The agarose gel showed that the mini-prep was successful, but we need to treat the DNA with RNAse.