- We performed PCR of 7 colonies of each construction (Jen1_orf and Jen1_promoter) inoculated yesterday.
We found 4 positive colonies for Jen1_promoter! =)
GEL
- Unfortunately no Jen1_orf construction was found in colonies selected but we continued screenig! To find positive colonies we screened 10 more colonies and made a backup inoculum in case we find right ones. Tomorrow we'll have the answer.
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