Culture growth of bacteria containing the F plasmid
From a permanent culture of bacteria containig the F plasmid, a sample was innoculated into liquid LB-AMP medium and let grown O/N at 37°C.
Gabriel
finO and finP's transformed bacteria
All of yesterday's transformed and plated cells grew in the media!
We then selected 10 colonies of each transformation (finO+pSB1A3 and finP+pSB1A3) in order to confirm them. We inoculated those colonies separately into liquid LB-AMP medium, and let then grow O/N at 37ºC.
We will then proceed to the minipreps of the cultures and, once we could make another PCR for the inserted fragments, we will be able to confirm that both finO and finP are indeed in biobrick format.
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