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ColiGuard
finO-biofusion - transformation results
- Only two colonies grew in yesterday's plates.
- Although we had experienced a low efficiency in biofusion usage, we won't continue finO's biobrick construction.
Marcelo
finOP - Final Conclusion
- After several unsuccessful attempts on constructing finOP's biobricks, using different strategies with different backbones, our team decided to leave aside those biobricks.
- Finalizing these parts will demand a lot of time, and we intend to already send our assembled biobricks this Thursday.
- It's quite possible to fully assemble them, but we haven't enough time right now. We got a lot of work to do related on characterizing our assembled biobricks and updating this wiki you are currently reading on.
- Thus, we will now focus on other kind of work. We are done with finOP inhibition system.
Marcelo
YeastGuard
pADH1+YFP (new biobrick)
Today we made the miniprep and the digestion of the plasmids containing our new briobrick (pDHA1+YFP). The enzymes used were XbaI and PstI.
Then, we performed the purification of the bands correspondig to the size of this new biobrick pADH1+YFP. So, it was sent to IGEM organization. =)
Wesley and Sula
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