Yesterday's transformed and plated cells grew in the media! We found white and blue colonies in the plate just as expected (due to the beta-galactosidase gene contained in pGEM vector).
We selected 10 white colonies (those theorically contains our inserts in the correct position, since it interrupts the coding sequence of beta-galactosidase) from each plate and inoculated them into liquid LB-AMP media.
Inocula were keep at 37ºC, under 250 rpm, for an O/N period.
Fabi, Leo, Marcelo and Victor
Transformation of the BBa K112806 + BBa B0015 ligation
We transformed the ligation made yesterday by eletroporation according to the Protocol 3
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