Today we performed a PCR of each September 15th's minipreps samples, using the finO and finP designed primers, in order to confirm that our inserts are correctly contained in the plasmid vector. Unfortunately, our lab ran out of Taq Polymerase, and we were forced to used a home-made Taq, which efficiency is much lower than the industrialized one.
Sadly, we weren't able to confirm finO and finP's biobricks, as our PCR's didn't amplified even a single fragment. However, that result may be a consequence of the home-made Taq usage, and we intend to repeat those PCR's with a trustable Taq.
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