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Overview Sensitivity Tuner --- Characterisation --- Modelling Colour Generators --- Carotenoids (Orange/Red) --- Melanin (Brown) --- Violacein (Purple/Green) The Future Safety

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Pigments

Though E. coli does not naturally produce pigment, several other bacterial species secrete pigmented antibiotics. We mined bacterial genomes for pigment-producing operons, and transformed the most prominent candidates into E. coli. In particular, we have devoted our summer to 3 different pigment systems:

• Carotenoids: The enzymes required for carotenoid production originally come from Pantoea ananatis, and were available in the registry. We used them to produce orange and red.
• Melanin: The tyrosinase required for melanin production originally comes from Rhizobium etli and produces brown.
• Violacein: The enzymes required for voilacein production originally come from Chromobacterium violacein. The operon can be manipulated to produce voilet, green, and blue.

We chose to pursue these 3 pigment systems for several reasons. Visual Diversity: Between these systems we've almost made -- as cheesey as it sounds -- all the colours of the rainbow.

A COOL PICTURE HERE

Design: We were able to use a variety of different techniques to build and design these pigment systems as biobricks. The carotenoid parts were already in the Registry, and were used standard assembly protocols to build pigment producing devices. The melanin system is a single gene which we endeavoured to turn into a biobrick by PCR. Finally, we designed the violacein operon for synthesis, codon optimizing it for both E. coli and B. subtilis and designing it so it is easy to manipulate to generate different pigments.