Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+Adh1-Lysozyme.
We inoculated the yeast pre inocula in 3 types of media: YNB Glucose Ura-, YNB Ethanol Ura- and YNB Glucose Ura+. The inocula grew for 4 hours in 30ºC and 150rpm before the induction with lactic acid. Four concentrations of lactic acid were used in the induction.