Team:IBB Pune/project/Results

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Revision as of 01:07, 22 October 2009 by Samitwatve (Talk | contribs)





We carried out the following transformations successfully:

Contents

Native Biobricks

  1. pLL promoter - BBa_I746365
  2. GFP coding sequence - BBa_E0040
  3. RFP coding sequence
  4. GFP Translational unit
  5. RFP Translational unit
  6. Enhanced CFP
  7. Fast GFP
  8. Constitutive promoter -BBa_J23102
  9. Promoter + RBS
  10. T7 promoter
  11. pLuxR
  12. Double terminator
  13. RBS
  14. Constitutive LuxI
  15. LuxR Translational unit

OUR PARTS

  1. YcdB
  2. TorA
  3. Lac Operator


Assembly

  1. Const. Promoter-LacO
  2. PluxR- LuxI
  3. {Promoter + RBS}-GFP
  4. {Promoter + RBS}-TorA
  5. {Promoter + RBS}-Ycdb
  6. luxR-PluxR-{RBS-LuxI}
  7. Promoter + RBS
  8. YcdB-GFP
  9. TorA-GFP
  10. {Promoter + RBS}-LuxR
  11. {Promoter + RBS}-YcdB-GFP
  12. {Promoter + RBS}-TorA-GFP
  13. Const. Promoter-LuxR


Failed Transformations

Backbones

  1. pSB1AK3 with CcdB gene
  2. pSB1T3 with CcdB gene
  3. pSB1C3 with CcdB gene
  4. pSB1K3 with CcdB gene
  5. pSB1A3 with CcdB gene
  6. pSB1AC3 with CcdB gene
  7. pSB1AK3 with mRFP
  8. pSB1T3 with mRFP
  9. pSB1C3 with mRFP
  10. pSB1K3 with mRFP
  11. pSB1A3 with mRFP
  12. pSB1AC3 with mRFP

Native Biobricks

  1. Lysis Cassette
  2. Constitutive GFP
  3. COnstitutive CFP

GEL PICTURES

10sepgel.jpg

14octgel.jpg
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